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A simulation study was used to estimate the effect of quantitative trait loci(QTL) and its location using flanking markers by regression method. Various parameter combinations such as heritability(0.05, 0.10, 0.30), map distance between markers were used to find the relative effects of these parameters on the estimation of the parameters using regression method. The true effect of QTL additive effect was assumed 0.3 phenotypic standard deviation. The relative size of map distance between flanking markers(10cM, 20cM, 40cM), assuming the QTL being located on the middle, was found no significant effect on the estimates of true map distance. And also, the various sample size per marker genotype(50, 100, 200) was not a significant factor on the parameter estimates. The main criteria for the estimation of the QTL effects and QTL location were the minimum mean square error from the regression model by iterating the map distance from 0cM to 50cM with a small size of interval(0.05 cM). The distribution of estimated error mean squares by the regression model by iterating the map distance from 0cM to 50cM with an interval of 0.05cM followed a smooth quadratic form. In all cases, the estimated QTL location was found exactly coincided with the true QTL location and the estimated additive genetic effect of QTL was quite accurate.
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